Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAANPPRPSRSLQGKVAIVTGAGSRGETIGNGRAIALLLAEDGAKVVCVDREEALAQRTAELVLAENEGASALAVAADVSSAEECARVVDAALRLFGRVDVLVNGVGVLGPPGTAVDVDAARWARGLEVNVTSMMLMAKYAVPAMRDNEPEAGVRGSIVNLGSVAGLRGGTPSLLYPTSKCAVVNMTRAMAAHHAKDGVRVNCVCPGMLYTPMVYDAEGGMSAEVREARRRRSLLQTEGNAWDCAGPVRFLAGGEARWITGTVLTVDAGATCALAVSMPDGE 5HA5 Chain:C ((4-250)) ------------LKEKLVLVTGAGR-----GLGAAISSGAAEQGARVILVDIDGTAAKAQADALTAK--GFVAEGHALDVTDRDAVAALADDILSRFGGLDVLVNNAGVAGRAAFDQPEAVEVWDRVIGVNLEGAFNVSHALVPALK------AAKGNVVHLCSVAGFVSGGSTAGYVVSKGAIRSLTQVMARDLAPHGIRVNAVAPGIMMS--------------TDWFMNRVMMKRIGETSEVVDPVVFLASPMASYITGTILPVDGGFLAA---------

General information: TITO was launched using: RESULT: Template: 5HA5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1396 -157183 -112.60 -668.86 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : -112.60 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.403

(partial model without unconserved sides chains): PDB file : Tito_5HA5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ha5-query.scw PDB file : Tito_Scwrl_5HA5.pdb: