Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNIKESVQKLLG--EELIVFELKGKGACNNAYYVETQSGKKFIIKEERPNGELKEQNSLAVEAHVIKQLSNL--NFSAPLAK-VYFISKEPNMFGYVYIEGDLLID-AWKKLTEEERIGICQNLGVFHAELEQRVTEAMAREMGVQINTYIGLHPEVE---EEYSKILTFTDIPN-GWKTLAQKTKTIFDK-TLELGVFQFLHNDAHHENIIVKDGEITGIIDFGDSEYGEIAREFSRYIRD----F-PDHAEYIIKAYEKASGHKLSRERLISNSFLSGLMDNVEDYRKGGEDCIRAEKAVSKYEDIFT 3HAM Chain:A ((1-278)) MVNLDAEIYEHLNKQIKINELRYL-SSGDDSDTFLCN--EQY-VVKVPKRDSV---RISQKREFELYRFLENCKLSYQIPAVVYQS-----DRFNIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKELHSIEIDCSVSL-FSD-ALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILNNAVLFKYTPCLVHNDFSANNMIFRNNRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGRKVLKYYQHKAP-EVAERKA-ELNDVYWSIDQIIYGYERKD-----------------

General information: TITO was launched using: RESULT: Template: 3HAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1108 35750 32.27 136.45 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 32.27 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.336

(partial model without unconserved sides chains): PDB file : Tito_3HAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ham-query.scw PDB file : Tito_Scwrl_3HAM.pdb: