Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MLKAKAERKFKKYFAQGLLALPYGQL----IEKIVPVLQAPKDSLEIRQIIDRPFSVIMEVVTDEGRSFFVK---------VYKLRNPEKQ----------RAELERE---FAVSRFWFDKFKDDPEHRV--IEPIWL-DVENLILITRKSEGENLLKLTGRLHLFPAKRVQ-----QRVHR-SLSQAGQWLKKFQS-----FSIVEEVPYVKPPVELTFSF--------------LQDYILIRLQRMVQNPKLDFDEHFQQKIIDYLKSLWQKAGAQSERLTFAHTDFSLSNILAAEDK--VTVLDFNKCEINSPYKDLSRFYHQLYLLSFKPTFQKSVIEEMKASFLQGYGAPLAKDHPMFQIFFIIHQ----VTHLGKISRFWERGLLENVYNHYL---VSRVLKDLKQ----------TVLV---------- 4EPQ Chain:A ((34-472)) DYQFPLPQKNSELWIIQKKTLQDLSSGKQKLDSFQSLESILEILRDSKNQNDEKYFNLKAVFEQLDKEEQTYFLEQFIPKICQLVLKIKKKQLKNQIPKESKIYEAAFSREEISYYVSCMFLCILKDQDRKIYKDFRLIYLKDLVQQINIRRQEKIKCFYEYLKQALDFSEKESKEVVIFQRINCGQLEDYENWVDKLKAIKLKNVQLTDDKLIEDFPGTLQVDFANCDIGGGILGNGLVQEEIRFCVCPEMLVSLLVFDQSMEANEVIIMKGIKQYSDYQGYSNSFRFVKMGNSKIQKQKRTNPQTILAIDALCFNSSDNQFSEVNVSRELNKSYMGFKQEDQLKTISTGKWGCGAFLGVFDLKFAIQWIASSRSNKKMIICTFQDEQTTKQIQQVFDLYKQKNASIFLKLVMDYPNSKYMEDYTLLEYLIELGKEKA

General information: TITO was launched using: RESULT: Template: 4EPQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1697 52105 30.70 147.60 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 30.70 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_4EPQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4epq-query.scw PDB file : Tito_Scwrl_4EPQ.pdb: