Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVSQPVRSAAEWTAGDVAGVLAQACPA-ARVTSIRPGPPSYSNCLWLAETVDGRLLVRIPGRSA-DPEYVRATVTATHLASAAGVPTVRYHEFVPQTPLGLPVTIQEYLPGE-SGTAALRADRADLRLLASTLGDWLGTLHGVRRETFGAVTGSGERTTWTAAATDQVETALRAVPEAALPASPATIAAAFARVVAELG-PGEPASLVHGDLYLDNMLVDN-GAPAALLDFEHAHYYDRFADFGKLSELLFEWWPGAEEPFLESYRDHFPPDPADEVRLRLGVGLYALNQTAYFARWQPELVGEYRTRLGKWLAAG 4R7B Chain:A ((21-274)) ------------MEKIIKEKISSLLSQEEEVLSVEQL-GGMTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIE-----AGIKVNEYIESAITLDST------SIKTKFDKIAPILQTIHTSAKELRGEFAP--FEEI--KKYESLIEEQIPY-------ANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRL-YLIDWEYSSMNDPMWDLAALFLESE-FTSQEEETFLSHYESDQT--PVSHEKIAIYKILQDTIWSLW-----------------------

General information: TITO was launched using: RESULT: Template: 4R7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1115 4129 3.70 16.58 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 3.70 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.337

(partial model without unconserved sides chains): PDB file : Tito_4R7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r7b-query.scw PDB file : Tito_Scwrl_4R7B.pdb: