Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNIKESVQKLLG-EELIVFELKGKGACNNAYYVETQSGKKFIIKEERPNGELKEQNSLAVEAHVIKQLSNL---NFSAPLAK-VYFISKEPNMFGYVYIEGDLLI-DAWKKLTEEERIGICQNLGVFHAELEQRVTEAMAREMGVQINTYIGLHPEVEEEYSKILTFTD-IPN-GWKTLAQKTKTIFDK-TLELGVFQFLHNDAHHENIIVKD-GEITGIIDFGDSEYGEIAREFSRYIRD----F-PDHAEYIIKAYEKASGHKLSRERLISNSFLSGLMDNVEDYRKGGEDCIRAEKAVSKYEDIFT 5IQC Chain:A ((15-288)) ---MKYLIEHYFDNFKVDSIEII-GSGYDSVAYLVN--NEY-IFKTKFSTNK---KKGYAKEKAIYNFLNTNLETNVKIPNIEYSYIS-DELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC-TID-NKQNVLE-EYILL---RETIYNDLTDIEKDYIES-FMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDI-EKAKEYQ-DIVEEYYPIETIVYGIKNIKQ----------------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1159 49342 42.57 190.51 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 42.57 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.363

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: