Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIQTVLSDLKLN--GKVIPVVGGDVNQTYRIKTE-HRAYFLKIHPNV-KKGFFEAEVDGLKELSAF--VRVPDTYMLGETS-EGAY----LLMEWIE-PG--K--------GDQRDLAAALANLHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQEKYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQ-GHPVFVDPA-VSYGNREQDIAMSQLFGG--FRPEFLDAYQTIFPLE-K----GWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLENF 5IGH Chain:A ((12-299)) --LYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAG--MLI--RTPTQARQKVADDV-----DRVRREFV----VND---KRLHRWQRWLDDDSSW-PDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQLAA-

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1112 -12423 -11.17 -48.34 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -11.17 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: