Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTETLTKELAVTKALELPFWKAPSNAEILGGGITNF---------NVKITDEGRTYVVRLGEDIVEHGVMRFNELSACRAAHAA-GVAPAVR-YFQKGALVQDYIPADPLVEEEIQQLKMLELIAALLGKVHRDATQLIRGPVLAFWVFHILRDY-TETLRL--LDSDHISKLDDLMQKAQKLEAAVGNVH---------LVLCHNDLLPANIL-------NENGRLWLVDWEYAGMNSPLFDLGGLATNAGLSEGLQKL------------------LLETYF--------------DTPVSDELWERYNAMKCASLLRESMWSMVSEQTSKIQFDYADYTRENLLRFERAFAELQLK 2CKO Chain:A ((66-384)) ---------------------------VIRGG-SNMLFQCSLPDTTATLGDEPRKVLLRLYGAI-------MVLESVMFAILAERSLGPKLYGIFPQGRLEQ-FIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPK---WLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLE--LENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQA---RFDAYFHQ----

General information: TITO was launched using: RESULT: Template: 2CKO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 862 -9947 -11.54 -39.95 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -11.54 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_2CKO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2cko-query.scw PDB file : Tito_Scwrl_2CKO.pdb: