Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence DCFIGLYEKGG--RNPNHIAGTE----ADESGTFEVLHENYKIRVK-IFKCVKQHVYDSTPSGTEIRVI 3ZPL Chain:E ((62-127)) --LVKLAESPRRRLRMTELAKYAKITRSRLSHAVARLEKNGWVRREDCPSDKRGQFAILTDEGYEV---

General information: TITO was launched using: RESULT: Template: 3ZPL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 172 15927 92.60 279.42 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain E : 0.68 3D Compatibility (PKB) : 92.60 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.164

(partial model without unconserved sides chains): PDB file : Tito_3ZPL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3zpl-query.scw PDB file : Tito_Scwrl_3ZPL.pdb: