Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDNAFTFQTLHPETIMDALFEQGIMVDSGLTPLNSYENRVYQ-FQDEDRRRFVVKFYRPERWSVDQIREEHQFALELVKDEVPVAAPLAFNGQTLLAHQGYHYAIFPSVGGRQFEAD-------NIDQMEAVGRYLGRLHQTGRKRPFTFRPDIGLAEYLFEPRQVFEDAALIPSGQKAAFLKATDTLLSAVTECWRTDFATLRLHGDCHAGNILWR-D-----GPLFVDLDDARNGPAIQDLWMLLNGDKA-EQRMQLETIIEAYEEVSEFDTAEIGLIEPLRAMRLVYYLAWLIRRWGDPAFPKNFPWLTGEDYWQRQTTTFIEQTKILHEPPLQLTPMY 3CSV Chain:A ((4-259)) ----------SREDEIRDFLATHGYADW-NRTP-------RYQRLRSPTGAKAVLMDWSPE--EGGDTQPFVDLAQYLRNLDISAPEIYAEE-------HARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLDPET-LSEMTRLAFSEYRYA-ILGDAAEDNRKRFEHRFAQILSAQ--LEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFR----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 971 -13472 -13.87 -57.82 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -13.87 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.264

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: