Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTAKTPEFSNAQVIELLLQQYKLSGQLKKLPGYCDQNLLLNTDDGKQYIVKIANAVEPKLELEMQNAAMAHLTHKQCAVPCAVNNIQGDNISIITDVSKQSFCLRVLTFIPGKFYADANSNTHSRALWTDLGQFIGNIDLALADFQHPGAFRYLDWDLAQGYRVCMSKKHLLNPQQVNLVDKFLSLYQTQTMPVLSQLPQGVIHNDANDYNLLIDDINTPNKISGLIDFGDMVHSHIVNELAIACAYALMGEKSVHEDILSALTSIVAGYHKIRPLLDVELEVLYSLIALRLCTTVCNAALAIQQQPDNEYLLVSVQPAWQLLEQLSAVNSYAVLCQLRLACQLPVDSGQSAAEIINYRKKHLGKTLSLSYQQPLKMVRGQGAYLFNEQGVPYLDMVNNVCHV--GHCHPKVVAAGQAQLAKLNTNTRYLHDNIVNYADKLLATMPDKLSVCMLVNSGSEANELAFRLARCFTKSRELLVVDGAYHGNTNA-CIEASP--YKFDGPGGEGAPAYVHKVTLPDPYRGEFLGNNSDAATAYANSVKETIEQLALNNKKPGAFICESLQGVAGQIIMPDGYLTQVYAHVRAAGGVCIADEVQVGFGRVGSHMWAFETQNVIPDIVTLGKPIGNGHPMAAVITTQEIADAFVT-GMEY-FNTFGGNPVSCAIGIAVLDVIEQEQLQNHALTTGQYFQDQLNKLKQRFELIGDVRGKGLFIGVELVEDRTTKQPATDKTSWLIEHFKQHHILLSTEGPFYNILKIKPPLAFNSGDADKFINVLELGLAVLSH 2E7U Chain:A ((35-318)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PPFLVRGEGAYVWDADGNRYLDYVMSWGPLILGHAHPKVLARVRETLER-GLTFGAPSPLEVALAKKVKRAYPF-VDLVRFVNSGTEATMSALRLARGYTGRPYIVKFRGNYHGHADGLLVEAGSGALTLGVPSSAGVPEEYAKLTLVLEY----------------NDPEGLREVLKRRGEEIAAIIFEPVVGNAGVLVPTEDFLKALH-EAKAYGVLLIADEVMTGF-RL-AFGGATELLGLKPDLVTLGKILGGGLPAAAYAGRREIMEKVAPLGPVYQAGTLSGNPLAMAAGLATLELLEE-------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2E7U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1507 -9890 -6.56 -35.70 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -6.56 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.117

(partial model without unconserved sides chains): PDB file : Tito_2E7U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2e7u-query.scw PDB file : Tito_Scwrl_2E7U.pdb: