Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTASVEVPQYVAEETTARWPNVGPQWVSEAPGELRQLCEQYGAI---PETVLP-ARYGLVVAVQAKDRELIMKGSPDP--DGPNQVKVMSALADLQVGPTILESFSTDT-------GFWTIMTRIKPGEPLRNLGASLAPPDKLATILRPLVNQPSPSSTL----PYIGDWLRDRLEDDALSDLAPGRTVASETERSEALSVLSELTD----AGAQGLCHGDTSPGNILTGENGKLYLIDPR-GMRGEAAYDVAVLGLKSAM-------TVSPETRVSDLAKAVGVDVGRAERWAAIALAARV 2PPQ Chain:A ((11-259)) --------------------------------ELRNFLTQYDVGSLTSYKGIAE---NSNFLLHTTKDPLILTLYEK-KNDLPFFLGLMQHLAAKG-LSCPLPLPRKDGELLGELSGRPAALISFLEGMWLRKP--EAKHCREVGKALAAMHLASEGFEIKRPNALSVDGWKVLWDK---SEERA---DEVEKGLREEIRPEIDYLAAHWPKDLPAGVIHADLFQDNVFFLGDELSGLIDFYFACNDLLAYDVSICLNAWCFEKDGAYNVTKGKALLEGYQSVRPLSE---------------

General information: TITO was launched using: RESULT: Template: 2PPQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 869 28013 32.24 130.90 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 32.24 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_2PPQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ppq-query.scw PDB file : Tito_Scwrl_2PPQ.pdb: