Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFEFLKDIKELEDVESVEKINKGYSKDEKFKIKLKNKDEYLLFRLSDISLYEQKKKEYEVISKFSKLGFEMSKPISFGICNDKKNVYMLLSWINGVDLSDVLP-----KLSSEEQYLLGRKAGKILKAIHSLKVEDKNYDDSLKIKLLDKIEKYESSDVRVENDSNFIEYVKRNVDKICGNYSYLHGDFHPSNLILMENNEIGVIDFNRWEIFDSYEEFYKLESFGIEFSIPYCVGQIDSYFEDDVPNEFWEIQAIYVACYTLYSIKWAEKFGEKEILGMKRRFYKT-------FENYENFKTIIPKWYSDYKNNKGY-SL------------------------------------------- 1JVP Chain:P ((1-298)) ----------MENFQKVEKIGEG-TYGVVYKARNKLTGEVVALKKIRVP--STAIREISLLKELNHPNI----VKLLDVIHTENKLYLVFEFLH-QDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHS--------------------------------------------------HRVLHRDLKPQNLLINTEGAIKLADFG----------LARAFVVTLWYR---APEILLGCKYYSTAVDIWSLGCIFAEMVT-RRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKW-ARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL

General information: TITO was launched using: RESULT: Template: 1JVP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 1013 -49695 -49.06 -220.87 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain P : 0.64 3D Compatibility (PKB) : -49.06 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.248

(partial model without unconserved sides chains): PDB file : Tito_1JVP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1jvp-query.scw PDB file : Tito_Scwrl_1JVP.pdb: