Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTARAEAGSALRRRFGTPDSRTSDELPNTTLDLLIGSSG--GALLAEIVGERGGEVVGWS-----VRQVDHRPGVSVTVTYRCDVRWGDGLRRETLGASIGRPKHHDAPGVRTLDVDGQ----PAAVWMFPDDPGLPALRTALDPAAVGRLLGHHPVETTLRAYRPTRRAVVEATTPGGRVFLKVLRPAKAEALRRRHLLLTGAGVPVPDVLDHRGDGLLVLAPLAGTTLRDAVRTGGLVPSPRELLDVLDRLPAELCDQPRRAAWSENADYYATLVGAALPAEADRAATLADRVLDRIAGLPDDGPTHGDFYEAQLLLTGGRLTGVLDVDSAGPGRRADDLACLVAHLETLSLMPGW-----------DGPRLRRLAREY--------AAAFAADLARRG-----------TDPAELNARIAGVLLSLATGPHRVQEPD--------WQAQTAAR--LS--AADAWLTPARH----------------------------GSRGLRYGDPMGRIVF-----MCGPAGAGKSTYARR----LEADGFV------------RLSIDAAAWAAG----HREQPLAEPVRSRFEREVQERLVALAADGADVVVDLSFWSRRMRDTYRALLAPLGVVPET---VYLATPRDVVLARVAARTGSHADDVVLDEA----TAALYSDHFEAPTADEGP----------LTVVTPSG-------------------------------------------- 5F7S Chain:B ((1-730)) MITHRPRGIEHPYARSLDQLYPAIPIAGQSLTIGATTSGPCSRMRCFVLWPEHEQVFDMSPVNGWQTSIPHLPDQDATYYFEALTLDG---RTETSESFPLTPSHWSAEPVGHIDIDGDRFIPDSPLWLVSS-AGTHRVKFALRIEGDEHVVGFG-----ERYDQLDQR--------GLRLDSVVFEQYKAQGKHHRTYL----PMPFAQVVNEAGRAWGFHVETTRRTWYDVAAT----VSDRILIEVDLGFEAEKTPVVRVNTWSGS----PTDVLNGFLDVAGRPAEMPEWIFG-------------------LWASGNEWNTQSLVMEQMDRHRNEGIPVSVVVIEAWSDEEGFTIFRDARYVPNQGQPHRGPDFTYPSDGAWPDPAGMIRELHERGIRVILWQIPLQKTDDDLGPEALAQGNALIASGHVVKEPDGTPYKNRGWWFPNALMPDLSTEAGRQWWTEQRRYLVEDLDIDGFKTDGGEHAWGSDLRYEDGRRGDEGNNLYPVNYARAYGDLLRSAGKYPVTFSRSGFTGSQAHGLYWAGDEDSTWEAFRSSITAGITAGACGILYWGWDLAGFSGPVPEAELYARAFAAATFMPIMQYHSEFHHHELPLRDRT---PWNVAEQTGCGELIDLARHYTRVREALRPYLVAQTRQCLQTGKPLMRAMFYDHADDPEIWAHPRQYMLGDELLINPVTAPGATTWTTYLPEGQWEDYWSGEVSEGGHLVTRAVGWDIIPVYRRVG

General information: TITO was launched using: RESULT: Template: 5F7S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3048 28656 9.40 53.56 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : 9.40 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.114

(partial model without unconserved sides chains): PDB file : Tito_5F7S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5f7s-query.scw PDB file : Tito_Scwrl_5F7S.pdb: