Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGRPCEIQLLASGQSNQTLRLRTD--TEDYVLKRWRYEQVFAVDRELEVSIQKELAQQALAPEVLAYDAKQGWFLQPYYQAPSLQQVTLSPLI-KATVLAETLSKIHNTRVDIPAW---------------SMSERVEHYLQQLRSV-NS--------A-----VAAQMSQKLVPMQPLLEDWLSYPVLCHNDLSMNHILMA---D-PIRIIDWEYAGIGHPLFDIASAIVVN---------ELSDQASVRLITEYEKI-A--HYQIDRQRLAQWCEFVEWLNKVWQHLFRHLS 2QG7 Chain:E ((110-432)) ----SLEFQIING-ITNILIKVKDMSKQAKYLIRLYGPKTDEIINREREKKISCILYNKNIAKKIYVFFTN--GRIEEFMDGYALSREDI-KNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGG-DSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMH-

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 917 11545 12.59 48.71 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain E : 0.66 3D Compatibility (PKB) : 12.59 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.392

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: