Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence GLKDCQVSLTATSEARRSF--------WDTQYADYNDDVEISAGRHKFV--------IHVEQGCGGSKVSGTIPPHHL 5JK7 Chain:H ((27-74)) -----------KSEAVRHFPRIWLHNLGQHIYETYGDTW---AGVEAIIRILQQLLFIHFRI----------------

General information: TITO was launched using: RESULT: Template: 5JK7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 33 -2805 -84.98 -87.64 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain H : 0.58 3D Compatibility (PKB) : -84.98 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.132

(partial model without unconserved sides chains): PDB file : Tito_5JK7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5jk7-query.scw PDB file : Tito_Scwrl_5JK7.pdb: