Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIQTVLSDLKLN--GKVIPVVGGDVNQTYRIKTEHRAYFLKIHPNVK--KGFFEAEVDGLKELSA-F-VRVPDTYMLGET----------SEGAYLLMEWIE-PG-------KGDQRDLAAALANLHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFK-DRLESQISLAEETNRWNVQRQEKYLRFKERVLKSV----EPKKITPRLLHGDLWSGNVFFDQQ---GHPVFVDPA-VSYGNREQDIAMSQLFGG-------FRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLENF 6EF6 Chain:A ((32-308)) ---QQALTHYDVSDNASLRLL-NLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHS-QRWTRPA--GFG---R-FSW--DWEHCLGDTPRWGRWQDAEG----VGASETALLTRAQDLLHRKLEEYGSG-PDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVF-------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1021 2437 2.39 10.41 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 2.39 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.322

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: