Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRPPTPTADTVRRLVRTLLKDGAADASGPEVRPAAPGADPVTWLVGGRHVLRLARDRDAAARQRRELRLRDLVRTHVPVAVP---VSVAQGEWAPGLAYTLDTRV-PGASGAEHDV---SAVGEADLAGLLTGLREVPARQAETLGVPRAAPRSLEALRAAAEPAAERLAGADEFDAARLRQLTRAAAVQLAAQSAPP---VLVHHALTGGHLVVSADGRVRAVLGWAGTVLGDPAEDIAGLALAVGSPAAVRAATLAGYGARPCLRGLWLARCDAVLQLAARLDGTGTAPLPRLRTRLRHAWEAILLERVTELRAEAGEDGERRA 5IGP Chain:A ((46-240)) ----------------------------------------------GRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQA---RQKVADDVDRVRREFVVNDKRLHRWQR----WLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG-------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 694 -28172 -40.59 -152.28 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -40.59 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.295

(partial model without unconserved sides chains): PDB file : Tito_5IGP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igp-query.scw PDB file : Tito_Scwrl_5IGP.pdb: