Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------MDPQNIANLPSQPTENRVLKANHKT-----------------PAARYNIKVLRALQHTLKGSPEADLASLLPSTYSKKLQSFKDSAVHPSTSATGSFDSKPSFLDNDVRSRIDAADQSMTLVQLSSELNGLLQPHKDLSTGLVNLLAN-SEV--LYESAWAASIMVFRVSEN-------------------LVVKVTVDERSTLNEHRSLAYLQQHQHRF--PAPRPHGVVRLGQFCLLFTSFIPGITLEKAWSRFDNMEKTA----------ISNQLDTLVATLRLIPFPE------------------------------NTTLGGVLGGGCK-DARRGIRLNSEPI-FNVKQFEDFIFAGS----ETASPLY-TNLLRSLMPSLPATCVFSHGDLRPANIMVCQREDGSW-RIVGIIDWESSGF-YPEY-WDCVKATNNLTPREQVDWYSFLPDQISPHRYPTQWLVDRLWD-RSMVNG----------- 5BP8 Chain:A ((6-520)) APREAEAAALLAATVADPWGLVAPSVYDTARLVSLAPWLDGHRERLGYLAKEQNQDGSWGAPDGYGLVPTLSAVEALLTELARTDSGAPHLSPDDLAAAC-ADGLGALRDGLLAGPVPDTIGVEFVAPSLLADINTRLAALTEQAPGKLGAWSGTTLTSPAPDLDGALLAGVREMTEQAPLPEKLWHTLEAVTRDGTRGARPHEGAPPHNGSVGCSPAATAAWLGAAPD-PAAPGVAYLRDVQARFGGPVPSITPIVYFEQAWVLNSLAASGLRYEAPAALLDSLEAGLTDEGIAAAPGLPSDSDDTAAVLFALAQHGRTHRPDSLMHFRRDGYFSCFGVERTPSTSTNAHILEALGHHVTVRPDDAGRYGAEIRMISDWLLDNQLPDGSWMDKWHASPYYATACCALALAEFGGPSARAAVDRAAAWALATQRADGSWGRWQG--TTEETAYMVQLLMRTRTPGSPGTVARSAARGCDALLAHDDPASYPGLWHDKDIYAPVTVIRAARLAALALGGA

General information: TITO was launched using: RESULT: Template: 5BP8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2087 65939 31.59 170.83 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 31.59 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.198

(partial model without unconserved sides chains): PDB file : Tito_5BP8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5bp8-query.scw PDB file : Tito_Scwrl_5BP8.pdb: