Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTASVEVPQYVAEETTARWPNVGPQWVSEAPGELRQLCEQYGAIPE---TVLPARYGLVVAVQAK-DRELIMKGSPDP-----DGPNQVKVMSALADLQVGPTILESFSTD------TGFWTIMTRIKPGEPLRNLGASLAPPDKLATILRPLVNQPSPSSTLP----YIGDWLRDRLEDDALSDLAPGR-TVASETERSEALSVLSELTD----AGAQGLCHGDTSPGNILTGENGKLYLIDPR-GMRGEAAYDVAVLGLKSAM--TVSPETRVSDLAKAVGVDVGRAERWAAIALAARV 1ZYL Chain:A ((14-265)) -------------------------------DTIMDALFEHGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEV-PVAAPVAFNGQTLLNHQGFYFAVFPSVGGRQFEAD--NIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRK---LFEDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRD--GPMFVDLDDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGL----------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 908 30032 33.07 133.48 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 33.07 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.321

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: