Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRLCVSDSEALVLLQSLPHVPQPVHIRRSSQGGEHLVWTVNDALVLRIPADQSNAEPLLRERRLLDLLRRNDKIAPVVPPCIDVGVWNPHGWQYALYHKATGVSVESDPQAVTNATEDDLVELLVGLRGVGVGDALGVGVPEGDEVDF--EKLRGNARAALDVLQKSQQLMELGEVVSLEKLDAPWFVRLGGSMRRVLSHADLKGEHVFLDAAGRVTGVI-DWSDAQVGCPSVEVAGLAVAVGARMAARVAKRAGYEADIVCKGVIMARCNGVVCLEAILRGADDS-----------PECLVRRQLGRAFEELDA 4CRU Chain:B ((4-273)) ----MLEREKIYQWINELSS-------------------------------------PETRENALLELSKKRESV-----PDLAPMLWHSFGTIAALLQEIVNIYPSINPPTLTAHQSNRVCNALALLQCVASHPETRSAFLAAHIPLFLYPFLHTVSKTRPFEYLRLTSLGVIGALVKTDEQEVINFLLTTEIIPLCLRIMESGSELSKTVATFILQKILLDDTGLAYICQTYERFSHVAMILGKMVLQLSKEPSARLLKHVVRCYLRLSDNPRAREALRQCLPDQLKDTTFAQVLKDDTTTKRWLAQLVKNLQE

General information: TITO was launched using: RESULT: Template: 4CRU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1130 -56200 -49.73 -219.53 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -49.73 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.285

(partial model without unconserved sides chains): PDB file : Tito_4CRU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4cru-query.scw PDB file : Tito_Scwrl_4CRU.pdb: