Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTARAEAGSALRRRFGTPDSRT------SDELPNTTLDLLIGSSGGALLAEIVGERGGE--VVGW----SVRQV--DHRP--GVSVTVTYRCDVRWGDGLRRETLGASIGRPKHHDAPGVRTLDV-DGQPAAVWMFPD----------------DPGLPALRTALDPAAVGRLLG------HHPVETTLRAYRPTRRAVVEATTPGG--RVFLKVLR--PAKAEALRRRHLLLTGAGVPVPDVLDHRGDGLLVLAPLAGTTLRDAVRTGGLVPSPRELLDVLDRLPAELCDQPRRAAWSENADYYATLVGAALPAEADRAATLADRVLDRI--------------AGLPDDGPTHGDFYEAQLLL-----TGGRLTGVLDVDSAGPGRRADDLACLVAHLETLSLMPGWDGPRLRRLAREYAAAFAADLARRGTDPAELNARIAGVLLSLATGPHRVQEPDWQAQTAARLSAADAWLTPARHGSRGLRYGDPMGRIVFMCGPAGAGKSTYARRLEAD-------GFVRLSIDAAAWAAGHREQPLAEPVRSRFEREVQERLVALAADGADVVVDLSFWSRRMRDTYRALLAPLGVVPETVYLATPRDVVL------ARVAARTGSHADDVVLDEATAALYSDHFEAPTADEGPLTVVTPSG----------------------- 1I2D Chain:A ((2-573)) --ANAPHGGVLKDLLARDAPRQAELAAEAESLPAVTL------TERQLCDLELIMNGGFSPLEGFMNQADYDRVCEDNRLADGNVFSMPITLDAS----------QEVIDEKKLQAGSRITLRDFRDDRNLAILTIDDIYRPDKTKEAKLVFGGDPEHPAIVYLNNTVK-EFYIGGKIEAVNKLNHYDYVALRYTPAELRVHFDKLGWSRVVAFQTRNPMHRAHRELTVRAARS-------------RQANVLIHPVVGLT------KPGDID-HFTRVRAYQALLPRY--PNGMAVLG--------LLGLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGSNSKGEDFYGPYDAQHAVEKYKDELGIEVVEFQMVTYLPD---TDEYRP-VDQVPAGVKTLNISGTELRRRLRS------------GAHI-----------------PEWFSYPEVVKILRESNPPRAT-----------------QGFTIFLTGYMNSGKDAIARALQVTLNQQGGRSVSLLLGDTVRHELS-SELGFTREDRHTNIQRIAFVATELTRAGAAVIAAPIAPYEESRKFARDAVSQAG-SFFLVHVATPLEHCEQSDKRGIYAAARRGEIKGFTGVD--------DPYETPEKAD---LVVDFSKQSVRSIVHEIILVLESQGFLERQ

General information: TITO was launched using: RESULT: Template: 1I2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2478 50418 20.35 106.37 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 20.35 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_1I2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1i2d-query.scw PDB file : Tito_Scwrl_1I2D.pdb: