Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSPAALITAAEKTWPDLDYRDPAIPRQGMDHHVAVLGD-HILRASVTDAYRAQAPTESAVLAELAPL--TDTRLPLVLRHTD--DWSFTLHPLIPGRALDAAHWGRLPRSERGQLTDQLASLLTALHSRDIAASPYRDVEPWFHGPAPNPAPRALPAKVDLLREQVTELDLDREDRRVIGEILAGMDDLLDRAHHAHRARLVHGDLYPAHLLWSSG-HGLGAIDFSDMNLGDPAVDFAHLSD-----ISPELPDEVLARTGLDHDPGILDRAWAYKRWDAVFLLVDHLRTGHT-GAATAHSLFDAARHPTRPGAR 5IQC Chain:A ((10-300)) -TNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTID-NKQNVLE--------EY-ILLRET-IYNDLTDIEKDYIESFMERLNAT---TVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYK----------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1252 -13219 -10.56 -47.38 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -10.56 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: