Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRLCVSDS-EALVLLQSLPHVPQ-PVHIRRSSQGGEHLVWTVN----DALVLRIPADQSNAEPLLRERRLLDLLRRNDKIAPVVPPCIDVGVWNPHGWQ----YALYHKATGVSVESDPQ-AVTNATEDDLVELLVGLRGVGVGDALGVGVPEGDEVDFEKLRGNARAALDVLQKSQQLMELGEVVSLEKLDAPWFVRLGGSMRRVLSHADLKGEHVFLDAAGRVTGVIDWSDAQVGCPSVEVAGLAVAVGARMAARVAKRAGYEAD-------IVCKGVIMARCNGVVCLEAILRGADDSPECLVRRQLGRAFEELDA 5IGI Chain:A ((2-297)) -TVVTTADTSQLYALAAR-HG-LKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNR--LPFAVPDWRVANAELV-AYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRT---PTQARQKVADDVDRVRREFVV-NDKRLHRWQRWLDD---DSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEE--GLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQL---------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1265 -4029 -3.18 -14.54 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -3.18 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.313

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: