Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNIPNFNSFVKVEPIHKGWSGDKKYYVEAENGERFLLRVSDISK-YKEKESEFEIMKKMSATG--MKMSLPISFGVCENEKSVYQLLTWCDGVEAKEA-LYNLSDEEQYIFGQKAAKILRQIETIDYKPASEE-WVI-------SYQERVKYYIELYRKC-GYTFDGD---EMVISYLQTGLQHIGERPTALMHNDFQTDNMVISPDGELYIIDFQMCGIADPYHVLTGVGVSA--MYSIPFAKGQIEEYFGRNVPEDFWEKYNHYMLAEMLYSFTVGVNMEEERENTLHMFDDEVERIKNNGSHIPKWYQKKI 3HAM Chain:A ((15-277)) ------QIKINELRYLSSGDDSD-TFLC----NEQYVVKVPKRDSVRISQKREFELYRFLENCKLSYQIPAVVYQ------SDRFNIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKELHSIEI-DCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILNNA-VLFKYTPCLVHNDFSANNMIFRNNRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGRKVLKYYQHK-APEVAERKAELNDVYWSIDQIIYGYERK--------------------------------

General information: TITO was launched using: RESULT: Template: 3HAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1020 -3490 -3.42 -14.24 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -3.42 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.315

(partial model without unconserved sides chains): PDB file : Tito_3HAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ham-query.scw PDB file : Tito_Scwrl_3HAM.pdb: