Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPDDDLARRIRAAVDAGELHGVTAEPGDPEIAPLGSGESYTAWRIGSG---------DQARVLRVPRRPPHEMPRSMEAEFEVLRRVPPE-LGTSAVALETGADSPLGAPYMVTTHVPGRALRAADW-NPRLATSLAHQIARLHVAFAAGPAPSAASVPSASEQGEELLTWWGRYHPETLTD------PR---VSA-LLPAWLRELTRLAPAFE-AVPTHPLIHGDVVATNVILGPDGVPRLIDFEWSGPGDTAKDLALIGGRVTGG------PW-----YLPMTPDDVAALVTEYSRYSRHAG----AIDPQQLLARRDVYELLDRLGNLLYCLSRPGEARYGRWADDLACNLTDRLAD 1NW1 Chain:B ((74-395)) --------------------------EHLRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPET--ESHLVAESVIFTLLSERHLGPKLYGIFS--------GGRLEEYIPSRPLSCHEISLAHMSTKIAKRVAKVHQLEVPIWKEPD--------YLCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHISLSKSPVTFCHNDLQEGNILLPK--RLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQV----------------------

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1002 1898 1.89 7.44 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : 1.89 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.306

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: