Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKRPKCSIVKSVASRYLE-NIISVEPVHKGTSTFVYRLITETGIFYI-RFLPEK---ASFASEVLVHNTLYEL---DVKVPKVIAFEHKDKETNLSVMIIEEIAGICLEDSD----SQLDFKEILRNAGKQLALIHTISVDGFGWIDKSSHIKLKGENQRFEDYFNLYLENDLQALCKYSFSNEEIKQIRRLMISAGSILNVEKAVLVHGDFDISHIFHK-DGRYTGIIDFGEVRGNNRLYDLATFAG-F--YQDRKLYSYLLKGYCEITPLSARDLYATELMALFIILRFLGKKVNTKFSNHWFKLAKKQLNHINNLDNVEQLLDITRH 5IQC Chain:A ((8-285)) --NATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVN----NEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYIS---DELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTID------NKQNVLEEY-ILLRETIYNDLTDI--EKDYIESFMERLN--ATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGN--IDIEKAKEYQDIVEEYYPIETIVYGIKN--------------------------------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1155 -7412 -6.42 -28.29 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -6.42 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.322

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: