Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLKSGEAEHETPHSKIPPDSHGIIPPDLQPKIPSFLSGTSRLDDSRQRPTADLPKRGFEEPIAQEVPESEAVSVPPSLAAWIRAQRGEIIADLSGELCECFVLRIRTEASGDV-VAKIPGKFRYPVERELRARPYLEGLINERLAPREV----FADSSQAVIVYDFVPGEAASKAT----AHLSAEKIPRIFRQAGYALARLHQAATRPSNGYEKAQVCRTLARIAEIEDSAFSTLRREHSELLSQAREELGAYQPRDLPLVPSHGDYRADNWIVGEGGELRVIDFG--RFGFRPALADFMPLARSEWPKNSLLRDVFLEGYASGGAGHGTIG------GI----IAEGRGGWWRVSNICDVLGNLWFEVRGASARLQAEDSDKRTAERSSEATRQAHPHAQADLQLDAQAELQAGFTALARALGF 1UWH Chain:A ((1-276)) ----------------------------------------------------------------------DDWEIPDG-----QITVGQRIG--SGSFG--TVYKG--KWHGDVAVKML--NVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTAPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKS--------------------------------------------------------IIHRDLKSNNIFLHEDLTVKIGDFGLATVLSGS----------ILWMAPEVIRM--------QDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECL-KKKRDERPL-----FPQILASIELLARSLPK

General information: TITO was launched using: RESULT: Template: 1UWH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1158 -15545 -13.42 -63.97 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -13.42 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.241

(partial model without unconserved sides chains): PDB file : Tito_1UWH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1uwh-query.scw PDB file : Tito_Scwrl_1UWH.pdb: