Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGRPCEIQLLASGQSNQTLRLRTDT---------EDYVLKRWRYEQVF-AVDRELEVSIQKELAQQALAPEVLAYDAKQGWFLQPYYQAPSLQQVTLSPLI-KATVLAETLSKIHNTRVDIPAW--SMSERVEHYLQQLRSV---N----SAVA----AQMSQKLVPMQPLLEDWLSYPVLCHNDLSMNHILMAD--------PIRIIDWEYAGIGHPLFDIASAIVVNELS------------------DQASVRLITEYEKI-AH-------------YQIDRQRLAQWCEFVEWLNKVWQHLFRHLS 5FUT Chain:A ((30-350)) ---DEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----GAEAMVLESVMFAILAERSLGPKLYGIFPQ--GRLEQFIPSRRLDTEEL-SLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAK--

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 794 -7947 -10.01 -33.96 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -10.01 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: