Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDAAVSFAKDFLAGGVAAAISKTAVAPIERVKLLLQVQHASKQI-TADKQYKGIIDCVVRIPKEQGVLSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDKRTQFWLYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKAGAEREFRGLGDCLVKIYKSDGIKGLYQGFNVSVQGIIIYRAAYFGIYDTAKGMLPDPKNTHIVISWMIAQTVTAVAGLTSYPFDTVRRRMMMQSGRKGTDIMYTGTLDCWRKIARDEGGKAFFKGAWSNVLRG-MGGAFVLVLYDEIKKYT 4C9G Chain:A ((24-309)) --------LIDFLMGGVSAAVAKTAASPIERVKLLIQNQDEMLKQGTLDRKYAGILDCFKRTATQEGVISFWRGNTANVIRYFPTQALNFAFKDKIKAMFGFKKEE--GYAKWFAGNLASGGAAGALSLLFVYSLDYARTRLAADS------RQFNGLIDVYKKTLKSDGVAGLYRGFLPSVVGIVVYRGLYFGMYDSL------------LASFLLGWVVTTGASTCSYPLDTVRRRMMMTSGQA---VKYDGAFDCLRKIVAAEGVGSLFKGCGANILRGVAGAGV-ISMYDQLQ---

General information: TITO was launched using: RESULT: Template: 4C9G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1069 -43717 -40.89 -166.22 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -40.89 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.187

(partial model without unconserved sides chains): PDB file : Tito_4C9G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4c9g-query.scw PDB file : Tito_Scwrl_4C9G.pdb: