Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPDRPEAEIEAMSALFARHLPGLAARSVTRLGEGMDHVAYEADGGLILRMSKEPDPARRADRIRREAALLALVAEISPLPVPRVILS-----DPDAGVLGYVKLPG-----RPLAERPVAEPGRLAPALGEFLTRLHLAPPERFSALAAPDTQPLTAWLAEAERHHRAVAAHLPAAARRRIEAFLGGAPPAEPRALAF-C--HNDLGAEHVL--AEGGTVTGVIDWSDAEIADPAYDLGLLYRD---LGPEVTASILAHYAAPWDDAAGRRAVFYARCALLEDLAYGLRTGPRRYAEAALAHLDRTFSAWG 5C4K Chain:A ((25-315)) -TFDQVEKAIEQL-------YPDFTINTIQISGKGNDCIAYEINRDFIFKF---PKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKIN--EDNKKIKKLLSRELKGPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYRMKEKYWSFEKIIYGKEYGYMDWYEEGLNEI--------

General information: TITO was launched using: RESULT: Template: 5C4K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1183 -11831 -10.00 -43.34 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -10.00 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_5C4K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5c4k-query.scw PDB file : Tito_Scwrl_5C4K.pdb: