Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTETLTKELAVTKALELPFWKAPSNAEILGGGITNF---------NVKITDEGRTYVVRLGEDIVEHGVMRFNELSACRAAHAA-GVAPAVR-YFQKGALVQDYIPADPLVEEEIQQLKMLELIAALLGKVHRDATQLIRGPVLAFWVFHILRDY-TETLRL--LDSDHISKLDDLMQKAQKLEAAVGNVH---------LVLCHNDLLPANIL-------NENGRLWLVDWEYAGMNSPLFDLGGLATNAGLSEGLQKL------------------LLETYF--------------DTPVSDELWERYNAMKCASLLRESMWSMVSEQTSKIQFDYADYTRENLLRFERAFAELQLK 2CKQ Chain:A ((66-384)) ---------------------------VIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG--------AMVLESVMFAILAERSLGPKLYGIFPQGRLEQ-FIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPK---WLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLE--LENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENS-KQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQA---RFDAYFHQ----

General information: TITO was launched using: RESULT: Template: 2CKQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 927 -11529 -12.44 -46.49 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -12.44 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_2CKQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ckq-query.scw PDB file : Tito_Scwrl_2CKQ.pdb: