Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMIWGDGTFVMASGQLLCFARLFLLVPQLSSDAPDTILPDILEAFERHQDLHRVCGSPLSARPQPGLTNRVFRLEAEKGTFFLRLPRLEAAGNIDRKAEARNLMLAAATGIAVPPVFCDAEAGILVTRAVKVS-GSPAALSEQPSYSLARALGEALGQLHGSGVTFEGLLDPDSVLQAQWQGLRDAVRCREEMTGLEIVLQPI-GA-ATADGAGPALVPSHGDPSPGNCLMSC-DQLWLIDWEFSGMSDPAWDLAYAVLEHEMSADEEQVFLQAYAATGAAVPEADRLCVMKAKCDAVSALWALEQVAAGRDEDVFLPFARQRRGRALRQLARLS 4R7B Chain:A ((18-304)) ---------------------------------GSHMEKIIKEKISSLLSQ--EEEVLSVEQL-GGMTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITL-DSTS---IKTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIP----YANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTSQEEETFLSHYESDQT-PVSHEKIAIYKILQDTIWSLWTVYKEEQGE---DFGDYGVNRYQRAVKGLASYG

General information: TITO was launched using: RESULT: Template: 4R7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1359 4006 2.95 14.15 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 2.95 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_4R7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r7b-query.scw PDB file : Tito_Scwrl_4R7B.pdb: