Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence DDYDCTVIFRNNHAPQRQPIVVHTYYSR----------DLPIELDGVRHTIQLSG-CTPEQSQIPQGYSVEHMT 5VAE Chain:F ((39-96)) ---PDQVYFEN-LIASGQ--TIHEWSSSWNYQGDRQVPSLPLLKRGRSYSLTRD-TSYPSES------------

General information: TITO was launched using: RESULT: Template: 5VAE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 47 -1440 -30.64 -32.73 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain F : 0.63 3D Compatibility (PKB) : -30.64 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.239

(partial model without unconserved sides chains): PDB file : Tito_5VAE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5vae-query.scw PDB file : Tito_Scwrl_5VAE.pdb: