Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPDDDLARRIRAAVDAGELHGVTAEPGDPEIAPLGSGESYTAWRIGSG--DQARVLRVPRRPPHEMPRSMEAEFEVLRRVPPE-LGTSAVALETGADSPLGAPYMVTTHVPGRALRAADW-NPRLATSLAHQIARLHVAFAAGPAPSAAS----VP-SASEQGEELLTWWGRYHPETLTDP-------RV-S-ALLPAWLRELTRLAPAFE-AVPTHPLIHGDVVATNVILGPD-GVPRLIDFEWSGPGDTAKDLALIGGRVTGG--PWYLPMTPDDVAALVTEYSRYSRHAGAIDPQQLLARRDVYELLDRLGNLLYCLSRPG---EARYGRWADDLACNLTDRLAD 2QG7 Chain:E ((109-450)) --------------------------DSLEFQIING-ITNILIKVKDMSKQAKYLIRLYGPKTDE-IINREREKKISCILYNKNIAKKIYVFFT-----N---GRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEE---LINQLIREIQPFYICSHINWGLWSLLQGMHSSDFDFINYGMTRLTASC-----

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1219 3866 3.17 13.85 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain E : 0.66 3D Compatibility (PKB) : 3.17 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.346

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: