Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSPAALITAAEKTWPDLD-YRDPAIPRQGMDHHVAVLG---D--HILRASVTDAYRAQAPTESAVLAELAPLTDTRLPLVLRHTD---DWSF----TLHPLIPGRALDAAHWGRLPRSERGQLTDQLASLLTALHSRDIAASPYRDVE----PWFHGPAPNPAPRALPAKVDLLREQVTELDLDREDRRVIGEILAGMDDLLDRAHHAHRARLVHGDLYPAHLLWSS-GHGLGAIDFSDMNLGDPAVDFAHLSD-ISPELPDEVLARTGLDHD---PGILDRAWAYKRWDAVFLLVDHLRTGH-TGAATAHSLFDAARHPTRPGAR 5IGH Chain:A ((8-297)) --DTSQLYALAAR-H-GLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWV----VPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA--------DDVDRVRR-----EFV--VNDKRLHRWQRWLDDD--SSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQL------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1176 -17212 -14.64 -64.46 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -14.64 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: