Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPDDDLARRIRAAVDAGELHGVTAEPGDPEIAPLGSGESYTAWRIGSGD---------QARVLRVPRRPPHEM-PRSMEAEFEVLRRVPPE-LGTSAVALETGADSPLGAPYMVTTHVPGRALRAADW-NPRLATSLAHQIARLHVAFAAGPAPSAASVPSASEQGEELLTWWGRYHPETLT-D----PRVSALL-PAWLRELTRLAPAFE-AVPTHPLIHGDVVATNVILGPD------GVPRLIDFEWSGPGDTAKDLALIGGRVTGGP-----------WYLPMTPDDVAALVTEYSRYSRHAG--------AIDPQQLLARRDVYELLDRLGNLLYCLSRPGEARYGRWADDLACNLTDRLAD 5FUT Chain:A ((30-349)) --------------------------DEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG------GAEAMVLESVMFAILAERSLGPKLYGIFP------Q--GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPK--------WLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQA----------------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 888 -36283 -40.86 -143.41 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -40.86 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.295

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: