Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGRPCEIQLLASGQSNQTLRLRTDTEDYVLKRWRYEQVFAVDRELEVSIQKELAQQ-AL-APEVLAYDAK-----QGWFLQPYYQAPSLQQVTL-----SPLI-KATVLAETLSKIHNTRVDIPAWS----MSERVEHYLQQLRSV-----NSAVAAQMSQKLVPMQPLLEDWLSYPVLCHNDLSMNHILMAD----PIRIIDWEYAGIGHPLFDIASAIVVN-ELSDQASVRLITEYEKIAHYQIDRQRLAQWCEFVEWLNKVWQHLFRHLS 3N4U Chain:A ((22-282)) ----INTIEISGEGNDCIAYEI---NRDFIFKFPKHSR-GSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHK-DIPTVLEKYRMKEKYWSFEKIIYGKEY----

General information: TITO was launched using: RESULT: Template: 3N4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 987 17969 18.21 76.79 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 18.21 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_3N4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3n4u-query.scw PDB file : Tito_Scwrl_3N4U.pdb: