Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MDMKLSDSKTELARRHNKVVYQDGN------RVVKVFNDQKP--------ASDVFNEALNTARIMGTDIKVSEVLEVSRIEGGEWNGSWAIANSYIPGRTLRDIASEVSYPDKNNPDLDKLQEFTEKMVDLQISVQNVKAPLLNRQKDKLARMVNSVKAIDPSTRYDLELRVDGLHNIGAVCHGDFVPSNIIVADDGELYICDWAHVTSGDPVVDAAQTYLLLKLRHPIWAESYLELYTRKADVAKQKIHYWVPVVAAAELARGR----KRDEDFLMAQVNAGDFQ-------------------------------------------- 1PHK Chain:? ((15-291)) FYENYEPKE---ILGRGVSSVVRRCIHKPTCKEYAVKIIDVTGGGSFSAEEVQELREATLKEVDILRKVS-GHPNIIQLKDTY--ETNTFFFLVFDLMKKGELFDYLTEKVTLS-----EKETRKIMRALLE-----------VICALHK------------------------------LNIVHRDLKPENILLDDDMNIKLTDFGFSCQLDPGEKLREVCGTPSYLAPEIIECSM--NDNHPGYGK-EVDMWSTGVIMYTLLAGSPPFWHRKQMLMLRMIMSGNYQFGSPEWDDYSDTVKDLVSRFLVVQPQKRYTAEEALAHPFFQQYV

General information: TITO was launched using: RESULT: Template: 1PHK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 969 16832 17.37 79.02 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 17.37 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.350

(partial model without unconserved sides chains): PDB file : Tito_1PHK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1phk-query.scw PDB file : Tito_Scwrl_1PHK.pdb: