Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLSPLQLAAIVSAGVPGIYPVGAEPVREDSDDFDSAIIADSTGRRWRVRAPRRPDASMRLEAEHLILQSFSPGLRARLPFAVPTVAGTVPYAGGNVFVYTHIPGTIYDVDQLAELSRREVAARRPS-LASIIAKDIATLHVMPEDIIYEADLPSYSSEQIRLRKNAELERAAATGKVPADLLAHWRAVLSPGPMWQFRPRVVHGDMGAENLVLHVTPTEARIVEVTGWSEVHVGDPAQDFAWLYSCQDIDFTDEAIEVYRQQMPQLPDAYLAQRANFYAEFAIAQWLTHTVEVGDRDGATHAVSMLLDIQDDLRASGELLGQEEVGPLVGPAE 5IGT Chain:A ((20-233)) -----------------GLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAM----LKNRLPFAVPDWR----VANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTE---RVSGMIDWSEARVDDPAIDMA--------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 859 -57301 -66.71 -269.02 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -66.71 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.290

(partial model without unconserved sides chains): PDB file : Tito_5IGT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igt-query.scw PDB file : Tito_Scwrl_5IGT.pdb: