Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEDVQAVRGYLERSGWLEGIREVRFLAAGEYNENHYVRASGGEYVLRINHGSQLGLGREQIGYEFSVLSAVAPSGV-TPRPYRVDPWP---EGLSGGTLLMDYLPGRPLDY--SHDLERAARILARVHALPPDDGLIRQDRAVLDIVAESEGLLDRYPDHP--RPEM-RDRLLAYRDDVLTLNERSRDFLASEPPVMVNTEVNSGNFIMSHMDGRHGKGDFLVDWEKAVVSTRHQDLGHFLAPTTTLWKTDTQLDREQREAFLSAYRRELLLLGAAAPDMDALREGSAILERTILLRALSWSHMAYCEYTRAERAIRNADTLAKIRQYLDNAECFLTWKE 2PPQ Chain:A ((9-288)) ---EDELRNFLTQYDV-GSLTSYKGIAE---NSNFLLHTTKDPLILTLYEK---K---NDLPFFLGLMQHLAAKGLSCPLPLPRKDGELLGELSGRPAALISFLEGMWLRKPEAKHCREVGKALAAMHLASEGFEIKRPNA--L-SVDGWKVLWDKSEERADEVEKGLREEIRPEIDYLAAHW------PKDLPAGVIHADLFQDNVFFLGD-----ELSGLIDFYFACNDLLAYDVSICLNAWCFEK--DGAYNVTKGKALLEGYQSVRPL-------SEAELEALPLLSRGSALRFFLTRLYDWLTTP------------------------------

General information: TITO was launched using: RESULT: Template: 2PPQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1232 -49168 -39.91 -185.54 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -39.91 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.305

(partial model without unconserved sides chains): PDB file : Tito_2PPQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ppq-query.scw PDB file : Tito_Scwrl_2PPQ.pdb: