Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVLASNVFVSDPFNSANDPKMPFLAEALNPIKVQKYLTKALFNQFKNIQLHAIRVIRHKLGRRCAIAYELTVDTQSLVIIGKARAKGL--DHHSYELQRSLWQAGFSEDSSDGIS-VPEPLGIIPE-------WQMWLQKKVPGTIATDLLP------------------GTEGVAIASKIAEAAHKLHQTGIFTRRCHTMSDELNILHERLPQVAQQYPQWEKRLEQILSKCDRLGANTPELSQCGIHRDFYGDQVIVNGEHLYLIDLDLYCQ-------GNPALDIGNFIAHITEYSLRI----------------LGNPNALADREAAMAERFIQFHGEA---IRLGIQSYTTLTLVRHIY-ISTQISDRSSFTEAILELCEQRLR 5JY7 Chain:L ((141-431)) -----------------------------------------------------------------------------DAIFKLFRRITPGVHPDIELNRVLARA--------GNPHVARLLGSFETEWEGEPYALGMVTEFAANSAEGWDMATTSTRDLFAEGDLYAEEVGGDFAGEAYRLGEAVASVHACLAHELGTEEVPFPADVMAQRLAAAVDAVPELREHVPQIEERYHKLAD--TTMTVQRVHGDLHLGQVLRTPKGWLLIDFEGEP-LDERRRPDTPVRDVAGILRSFEYAAHQRLVDQAGDDDDRARQLAARAREWVTRNCASFCDGYAAEAGTDPRDSADLLAAYELDKAVYEAAYEARHRPSWLPI-------------

General information: TITO was launched using: RESULT: Template: 5JY7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 876 -2764 -3.15 -11.86 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain L : 0.64 3D Compatibility (PKB) : -3.15 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.292

(partial model without unconserved sides chains): PDB file : Tito_5JY7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5jy7-query.scw PDB file : Tito_Scwrl_5JY7.pdb: