Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHDALITAALHPYGLS--GAPFTVLRT-LGNVVARVDTPD-GPRGLRVCRSSVTRERLHEEFRVLAAARA-AGLDVPEPLPDVTGDPLPRAQGRDVALFRWVPG-EGAGAHM-----------SEAVAAQLGTLSAHLHAALRALGQPHPWYGPTHDAAWLSGPASWWATRADRD-LGAHACARMAPAIAACAQHLAHHAAEMQVIHADLHFGNALVRADGTVAAIDFDMCAVGLPAFDLAVTEGEFEDFDHPDALKAAFRGAYAAASGAPYPQGVALMRVAASVAFLEWVFTSANDEVRTQKLRWVPRLIDRVAARAPGARQGGPTGSAGPRP 5UXD Chain:A ((7-255)) ---LDALLDLAARHGLDLDGGTLRTEEIGLDFRVAFARAHDGGDWVLRLPRRPDVLERAAVEGRLLAMLAPHLDVAVPDWRIST----------SELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEVR--SPAQVR---GAWRQDLARVGAEFEIAPALRERWEAWLADDGCWPGHSVLTHGELYPAHTLVEDERITAVLDWTTAAVGDPAKDLMFHQVSA-----PSAIFEVALQAYAEGGGRP---------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 923 -24179 -26.20 -104.67 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -26.20 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.205

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: