Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPDDDLARRIRAAVDAGELHGVTAEPGDPEIAPLGSGESYTAWRIGSGD-QARVLRVPRRPPHEMPRSMEAEFEVLRRVPPE-LG--TSAVALETGA-DSPLGAPYMVTTHVPGRALRAA---DWNPRLATSLAHQIARLHVAFAAGPAPSAASVPSA---SEQGEELLTWWGRYHPETLTDPRVSALLPAWLRELTRLAPAFEAVPTHPLIHGDVVATNVILGPD-GVPRLIDFEWSGPGDTAKDLALIGGRVTGGPWYLPMTPDDVAALVTEYSRYSRHAGAIDPQQLLARRDVYELLDRLGNLLYCLSRPGEARYGRWADDLACNLTDRLAD 5IGI Chain:A ((25-280)) ----------------------------GPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEV--SAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFV------VNDKRLHRWQRWLDDDS---SWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMV---------FGEEGLAKLLLTYEAAGGRV----WPRLAHHIAERLAFGAVTYALF---------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1062 -28093 -26.45 -115.61 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -26.45 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.292

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: