Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRSRSRSSSRSKHTKSSKHNKKRSRSRSRSRDKERVRKRSKSRESKRNRRRESRSRSRSTNTAVSRRERDRERASSPPDRIDIFGRTVSKRSSLDEKQKREEEEKKAEFERQRKIRQQEIEEKLI-----EEETARRVEELVAKRVEEELEKRKDEIE--REVLRRVEEAKRIMEKQLLEELERQRQAEL---------AAQKAR-------EEEE-------------------RAKREELERILEENNRKIAEAQAKLAEEQLRIVEEQRKIHEERMKLEQERQRQQKEEQKII-LGKGKSRPKLSFSLKTQD 6FHS Chain:J ((75-764)) -----------------------------------------------------------------------------------------------------EDRERIVQYPYTEEVVVQKTEEELARIAERKKESGRRLQEQAAKMRLERLMKKEQELEYYKDIQRRMQGESKKEIKRLLDEAELKDEAALERVIRDLERSIKRARQKDLGEPEEEEVPDFSLLDVPDDQLDEAGLRQKRQ--QRLLKSNWEARQRAKAEKEAEKARLAEEAR-LDEERRKNDLEGWLEEKRQLRLAKLNQLKERERLKAD-----

General information: TITO was launched using: RESULT: Template: 6FHS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 563 102297 181.70 623.76 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain J : 0.73 3D Compatibility (PKB) : 181.70 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.126

(partial model without unconserved sides chains): PDB file : Tito_6FHS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6fhs-query.scw PDB file : Tito_Scwrl_6FHS.pdb: