Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLSHLVDVVRRAHPDVDLEGAGVHSGQFHDVL---IARDRVFRFPKTAGAAAELPGRVAVLTAVDAVELGVGVPVPLSEV-RDGGPH-----GFLVLSRLHGTPLERGDATS--PEVIDVVAAEFARVLRAMAGADVE--KLRLVLPV-----ADAGRWRGFAGR-VRATLFPLMSEDGRARAERELAAAVAMDHVATGLVHGDLGGENVLWQQVEELPRLTGIVDWDEAKVGDPAEDLAAV---GASYGPELVERVVALLGAGDL---WPRIRAYQGTFALQQALAGAEDGDDEELEDGLTAYR 4DT8 Chain:A ((5-296)) -TFDQVEKAIEQLYPDFTINTIEI-SGEGNDCIAYEINRDFIFKFPKHSRGSTNLFNEVNILKRIHN-----KLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDILENEIYFKYY-----PCLIHNDFSSDHILFDT--EKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYRMKEKYWSFEKIIYGKEYGYMDWYEEGLNEIR

General information: TITO was launched using: RESULT: Template: 4DT8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1167 11436 9.80 42.83 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 9.80 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.402

(partial model without unconserved sides chains): PDB file : Tito_4DT8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4dt8-query.scw PDB file : Tito_Scwrl_4DT8.pdb: