Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGISNTNPPGGAPRPRQQCFLSADLGPDV-MGQPTLLEATPFVNDMFDFHGYRCQMGKDCSHVRCRGFPAAFKMTAR--------------------------------------------------------------------------------------------------------------------------------------- 1HM9 Chain:A ((11-468)) SNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQPEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGDTPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATDFEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLKDFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEANVILKGQTKIGAETVLTNGTYVVD--STIGAGAVITNSMIEESSVADGVTVGPYAHIRPNSSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKYKTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYATRLPHHPKNQ

General information: TITO was launched using: RESULT: Template: 1HM9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 293 4173 14.24 56.39 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : 14.24 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.165

(partial model without unconserved sides chains): PDB file : Tito_1HM9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1hm9-query.scw PDB file : Tito_Scwrl_1HM9.pdb: