Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLKSGEAEHETPHSKIPPDSHGIIPPDLQPKIPSFLSGTSRLDDSRQRPTADLPKRGFEEPIAQEVPESEAVSVPPSLAAWIRAQRGEIIADLSGELCECFVLRIRTEA---------SGDVVAKIPGKFRY---PVERELRARPYLEGLINERLAPREVFADSSQAVIVYDFVPGEAASKATAHLSAEKIPRIFRQAGYALARLHQAATRPSNGYEKAQVCRTLARIAEIEDSAFSTL-----RREHS---ELLSQAREELGAY---QPRDLPLVPSHGDYRADNWIVGEGGELRVIDFGRFGFRPALADFMPLARSEWP-----------------KNSLLRDVFLEGYASGGAG--HGTIGGIIAEGRGGWWRVSNICDVLGNLWFEVRGASARLQAEDSDKRTAERSSEATRQAHPHAQADLQLDAQAELQAGFTALARALGF 3C5I Chain:D ((36-332)) ----------------------------------------------------------------------------------------LRVKQIL--L-TNQLFEVGLKEETANNYNSIRTRVLFRIYGKHVDELYNTISEFEVYKTMSKY--KI-APQLLNTFN--GGRIEEWLYGDPLRID-----DLKNPTILIGIANVLGKFHTLSRKRHLPEHWDRTPCIFKMMEKWKNQLFKYKNIEKYNCDIHKYIKESDKFIKFMKVYSKSDNLANTIVFCHNDLQENNIINTN-KCLRLIDFEYSGFNFLATDIANFFIETSIDYSVSSYPFFEIDKKKYISYENRKLFITAYLSNYLDKVVPT-PKLIDEILEAVEVQALGAHLLWGFWSIIRGYQ----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3C5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 912 -4896 -5.37 -19.51 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 0.61 3D Compatibility (PKB) : -5.37 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.241

(partial model without unconserved sides chains): PDB file : Tito_3C5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3c5i-query.scw PDB file : Tito_Scwrl_3C5I.pdb: