Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTASVEVPQYVAEETTARWPNVGPQWVSEAP----GELRQLCEQYGAIPETVLPARYGLVVAVQAKDRELIMKGSPDPDGPNQVKVMSALADLQVGPTILESFSTDTGFWTIMTRIKPGEPLRNLGASLAPPDKLATILRPLVNQPS--PSSTLPYIGDWLRDRLEDDALSDLAPGRTVASETERSEALSVLSEL--------TDAGAQGLCHGDTSPGNILTGENGKLYLIDPRGMRGEAAYDVAVLGLKSAMTVSPETRVSDLAKAVGVDVGRAERWAAIALAARV 5DKO Chain:A ((17-261)) TQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFFRNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVLISAP------------RIGQFLRE--------DRLIALVRQRLSIPGGCCSFDLPTLHIWLH---LPQAQRDSQVETWIASLNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGHKSRFAIRFMPLDTENGQVPERLDFELACC--------------------

General information: TITO was launched using: RESULT: Template: 5DKO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 790 7201 9.11 31.17 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 9.11 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.224

(partial model without unconserved sides chains): PDB file : Tito_5DKO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5dko-query.scw PDB file : Tito_Scwrl_5DKO.pdb: