Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLKSGEAEHETPHSKIPPDSHGIIPPDLQPKIPSFLSGTSRLDDSRQRPTADLPKRGFEEPIAQEVPESEAVSVPPSLAAWIRAQRGEIIADLSGELCECFVLRIRTEASGDVVAKIP--GKFRYPVERELRARPYLEGLINERLAPREVFADSSQAVIVYDFVPGEAASKAT-AHLSAEKIPRIFRQAGYALARLHQ----------AATRPSNGYEKAQVCRTLARIAEIEDSAFSTLRREHSELLSQAREELGAYQPRDLPLVPSHGDYRADNWIVGEGGELRVIDFGRFGFRPALADFMPLARSEWPKNSLLRDVFLEGYASGGAGHGTIGGIIAEGRGGWWRVSNICDVLGNLWFEVRGASARLQAEDSDKRTAERSSEATRQAHPHAQADLQLDAQAELQAGFTALARALGF 3HAM Chain:A ((1-299)) -----------------------------------MVNLDAEIYEHLNKQIKINELRYLSS----------------------------------GDDSDTFLC------NEQYVVKVPKRDSVRISQKREFELYRFLENCKLSYQIPAVVY--QSDRFNIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKELHSIEIDCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILNNAVLFKYTPCLV---HNDFSANNMIFRNNRLFGVIDFGDFNVGDPDNDF-LCLLDCSTDDF--GKEFGRKVLKYYQH----------------------KAPEVAERKAELNDVYWSIDQIIYGYERKDREMLIKGVSELL---------------QTQAEMFIF

General information: TITO was launched using: RESULT: Template: 3HAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1252 25674 20.51 89.77 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 20.51 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.225

(partial model without unconserved sides chains): PDB file : Tito_3HAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ham-query.scw PDB file : Tito_Scwrl_3HAM.pdb: