Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAG-HDFFVKCDEREL---LPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHSAFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAAEKGIAFGNIDAIVEHIQQRL---ASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDRECDLAMLPLH-TEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIAQQSLDRLLAA 3F7W Chain:A ((2-287)) -VNSVAARVTELTGR-EVAAVAERGHSHRWHLYRVELADGTPLFVKALPDDAPALDGLFRAEALGLDWLGRSFGSPVPQVAGWDD----RTLAMEWVDERPPTPEAAERFGHQLAAMHLAGAE-SFGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAADRGALTPGDVRLVEKVLDALDHLAGDPEPPARIHGDLWNGNVLWQDDGAVVIDPAAHGGHREADLAMLALFGLPYLDRVRDAYNEVAPLAEGWRARIPLHQLHPLLVHVCLFGAAYRTTLVDTARAALR-

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1496 -42589 -28.47 -153.20 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -28.47 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: